LOC_Os05g44340

Expression Profile

RGAP LOCUS ID LOC_Os05g44340
RAP-DB ID Os05g0519700
Function heat shock protein 101, putative, expressed

Sub-cellular Localization Predictions

1) WoLF-PSORT Prediction
localization cyto
score 11

2) CELLO Prediction
localization Cytoplasmic
score 2.384

3) NUCPRED Prediction
localization Non Nuclear
score 0.42

4) Y-Loc Prediction
localization Nuclear
score 99.88
confidence value 0.75
Number Of Software Predicting Nucleus 1
Seed Specific No
Transcription factor category

Experimental evidence for subcellular localization
Published gene name (updated 1 January 2020) HSP101|OsClpB-cyt|HSP100
Function assigned as per literature OsClpB-cyt gene is one of the early heat stress genes which encodes a chaperone protein that mediates disaggregation of denatured proteins
Subcellular localization as per literature Cytoplasm but upon interaction with OsHsfA2c, OsHsfA2c:OsClpB-cyt complex is localized in nuclei
Cells used for localization experiment Onion epidermal cells
NUCLEAR or Not Nuclear NUCLEAR
PMID 22147560
Reference of localization https://link.springer.com/article/10.1007%2Fs12192-011-0303-5
Is Subcellular localization evidence by author available ? No

Sequence Analysis
Number of PAT4 1
Number of PAT7 0
Number of Bipartite 1
Basic residues % 0.143
NLS score 0.27
Protein Sequence >LOC_Os05g44340.1 protein
MNPDNFTHKTNEALVAAHEIASEAGHAQLTPLHLVAALAADKGGILRQAISQASGGDAGAPDSFERVVSGALKKLPSQSPPPDSVPASTALIKVIRRAQS
AQKKRGDSHLAVDQLLLGLLEDSLISDCLKEAGVSAARVRAELEKLRGGEGRKVESASGDTNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRR
TKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKVILFIDEIHLVLGAGRTEGSMDAAN
LFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVFVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALVVAAQLSARYIMGRHLPDKAIDLVDEA
CANVRVQLDSQPEEIDNLERKRIQLEVEHHALEKEKDKASKARLVEVKKELDDLRDKLQPLTMKYRKEKERIDEIRKLKQRREELQFTLQEAERRMDLAR
VADLKYGALQEIDVAIAKLESETGENLMLTETVGPEQIAEVVSRWTGIPVTRLGQNDKERLVGLADRLHQRVVGQAEAVSAVAEAVLRSRAGLGRPQQPT
GSFLFLGPTGVGKTELAKALAEQLFDDENLLVRIDMSEYMEQHSVARLIGAPPGYVGHEEGGQLTEQVRRRPYSVILFDEVEKAHVAVFNTLLQVLDDGR
LTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGKNSMKVARDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKVARLQMKDVAVRLAERGVALAVTD
AALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKDELAYRVDNRGGLVNAETGQKSDILIQVPNGAATGSDAAQAVKKM
RIMEDEDGMDEE

GO Analysis
1
GO Category Biological Process
GO Term response to abiotic stimulus
2
GO Category Molecular Function
GO Term nucleotide binding
3
GO Category Biological Process
GO Term response to stress
4
GO Category Biological Process
GO Term protein metabolic process
5
GO Category Biological Process
GO Term cellular process
6
GO Category Molecular Function
GO Term protein binding
7
GO Category Biological Process
GO Term catabolic process
8
GO Category Biological Process
GO Term nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
9
GO Category Molecular Function
GO Term hydrolase activity
Presence of Splice variants No